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首页> 外文OA文献 >Extensive genetic variability linked to IS26 insertions in the fljB promoter region of atypical monophasic variants of salmonella enterica serovar typhimurium
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Extensive genetic variability linked to IS26 insertions in the fljB promoter region of atypical monophasic variants of salmonella enterica serovar typhimurium

机译：广泛的遗传变异性与沙门氏菌肠型鼠伤寒沙门氏菌的非典型单相变体的fljB启动子区域中的IS26插入有关

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摘要

Fifty-nine monophasic Salmonella enterica serovar Typhimurium isolates, collected in Belgium during the period from 2008 to 2011, have been serotyped as 4,[5]:i:− and shown to harbor an fljB coding sequence. The genetic differences between these strains and phenotypically biphasic Salmonella Typhimurium were analyzed through PCR and DNA sequencing. Genetic alterations in the fljB promoter region affecting expression of the phase 2 flagellin were observed in 53 isolates. Other genetic events in the invertible region carrying the fljB promoter were observed in 2 isolates. For the remaining 4 isolates, no molecular differences with a reference biphasic Salmonella Typhimurium strain could be observed. Next-generation sequencing of one representative isolate affected in the fljB promoter region revealed a 26-kb IS26 composite transposon insertion along with a local genomic rearrangement. Several other IS26 element-mediated alterations of this genomic region were observed. This group of monophasic Salmonella Typhimurium isolates was genetically heterogeneous, as revealed by multilocus variable-number tandem-repeat analysis (MLVA), PCR, and sequencing. Pigs and pork represented a major source of such monophasic isolates in Belgium, as reported in other countries. Three out of 5 isolates of human origin presented genetic profiles identical to those of food isolates, demonstrating the pathogenic potential of the newly characterized variants and potential dissemination along the food chain. This study highlighted the key role played by IS26 insertions in the loss of phase 2 flagellin expression and the subsequent generation of multiple monophasic variant lineages from biphasic Salmonella Typhimurium ancestors.
机译：在比利时于2008年至2011年期间收集的59株单相肠炎沙门氏菌鼠伤寒沙门氏菌的血清型为4，[5]：i：-，并显示其具有fljB编码序列。通过PCR和DNA测序分析了这些菌株与表型双相鼠伤寒沙门氏菌之间的遗传差异。 fljB启动子区域的遗传改变影响了2期鞭毛蛋白的表达在53个分离物中。在两个分离株中观察到了携带fljB启动子的可逆区域的其他遗传事件。对于其余的4个分离株，与参考双相鼠伤寒沙门氏菌菌株没有观察到分子差异。受影响的fljB启动子区域中的一个代表性分离株的下一代测序揭示了26 kb IS26复合转座子插入以及局部基因组重排。观察到该基因组区域的其他几个IS26元素介导的改变。如多位点可变数串联重复分析（MLVA），PCR和测序所揭示的，这组单相鼠伤寒沙门氏菌在遗传上是异质的。如其他国家所报道，猪和猪肉是比利时此类单相分离株的主要来源。在人类起源的5个分离株中，有3个具有与食物分离株相同的遗传特征，证明了新近鉴定的变体的致病性和在食物链中的潜在传播。这项研究强调了IS26插入在2期鞭毛蛋白表达的丧失以及随后由双相鼠伤寒沙门氏菌祖先产生的多个单相变体谱系中发挥的关键作用。

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